Mushrooms School
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Clone a Mushroom: Cardboard Spawn
Lesson 4

Build Your Spawn Sandwich

Clone a Mushroom: Cardboard Spawn▾
  1. 1What Is Cloning?
    1. What Is Cloning?9 min
  2. 2Save Your Stem Butts
    1. Save Your Stem Butts8 min
  3. 3Why Cardboard Works
    1. Why Cardboard Works10 min
  4. 4Build Your Spawn Sandwich
    1. Build Your Spawn Sandwich11 min
  5. 5Watch It Colonize
    1. Watch It Colonize10 min
The video for this lesson is being prepared.

Building day

Today you bring the two halves together: your fresh stem butts and your clean, damp, corrugated cardboard. You are going to build a spawn sandwich — cardboard on the outside, living clone tissue in the middle. Then you tuck it away and let nature do the rest.

Start the way every good grower does: wash your hands. Clean hands are your best tool against unwanted molds. Work on a clean surface, too.

What you will need

  • Your damp, pasteurized corrugated cardboard, now cool.

  • Your fresh oyster stem butts.

  • Clean scissors (a grown-up can help with cutting).

  • A clean cardboard tube, like the inside of a toilet roll.

  • A container or zip bag that closes.

Step 1: cut the tissue small

Take your stem butts and snip or tear them into small pieces. Smaller pieces mean more edges touching the cardboard, and every touching edge is a place where the mycelium can leap across and start feeding.

More pieces also give your clone a head start. The more living oyster tissue you spread out, the better your chances of out-growing any stray mold.

Step 2: layer the sandwich

Now assemble it, like making a real sandwich:

  1. Lay down a sheet of the corrugated cardboard as your bottom slice.

  2. Spread your stem-butt pieces evenly across it. Do not pile them in one corner — spread them out.

  3. Lay another sheet of cardboard on top as the lid. The living tissue is now hugged between damp cardboard on both sides.

The closer the contact, the faster the jump. You want the pieces pressed gently between the layers, touching the fibres.

Step 3: roll and tuck

Starting from one edge, roll the sandwich up into a snug spiral — firm enough for good contact, but not squeezed flat, because the mycelium still needs those air tunnels open. Slide the roll inside the cardboard tube so it cannot unravel. If you are using a flat container instead, just stack the layers and close them in.

Step 4: the cosy incubation spot

Place your roll into its container or bag and close it so it stays damp, then set it somewhere:

  • Dark — mycelium does not need light to spread; darkness helps it focus on growing.

  • Warm — around 20 to 24 degrees Celsius is ideal, like a comfy room. A cupboard is perfect.

  • Still — leave it be. No poking every hour.

Do not worry about the closed container. At this stage the fungus is not making spores or releasing anything — it is quietly building mycelium, and it just needs damp, warmth, and a little trapped air.

Patience is an ingredient

Nothing will look different for the first few days, and that is completely normal. Under the surface, the clone is waking up and reaching its first threads into the cardboard. A real grower knows that waiting is part of the recipe.

The same rules still hold: this is a known, cultivated oyster mushroom, we never use wild fungi, and nothing gets eaten until it is grown into fresh mushrooms and cooked by an adult.

Sandwich built and tucked away? Brilliant. Next you become a mycelium detective and watch it colonize.

Quick quiz

Answer every question, then check how you did. You can try as often as you like — your score is just for you.

1.Why do you cut the stem butts into small pieces?
2.How should you spread the stem-butt pieces on the cardboard?
3.How tightly should you roll the spawn sandwich?
4.What conditions does the roll need while it grows?

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